Recombinant人SGK1 protein (ab85649)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 氨基酸60 to 431

技术指标

Our Abpromise guarantee covers the use of ab85649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • OTTHUMP00000017247
    • Serine/threonine protein kinase SGK
    • Serine/threonine protein kinase Sgk1
    • Serine/threonine-protein kinase Sgk1
    • Serum and glucocorticoid regulated kinase
    • Serum/glucocorticoid regulated kinase
    • Serum/glucocorticoid regulated kinase 1
    • Serum/glucocorticoid-regulated kinase 1
    • SGK
    • SGK 1
    • SGK1
    • Sgk1 variant i3
    • SGK1_HUMAN
    see all
  • 功能Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
  • 组织特异性Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 结构域Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
  • 翻译后修饰Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
    Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
  • 细胞定位Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
  • Information by UniProt

Recombinant Human SGK1 protein 图像

  • SDS-PAGE showing ab85649 at approximately 72kDa.

Recombinant Human SGK1 protein (ab85649)参考文献

ab85649 has not yet been referenced specifically in any publications.

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