重组人SFRP1蛋白(ab64445)
Key features and details
- Expression system: HeLa cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人SFRP1蛋白
参阅全部 SFRP1 蛋白酶 -
生物活性
Biological Activity :Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3-0.5 µg/ml.
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纯度
> 95 % SDS-PAGE.
Purity: Greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
表达系统
HeLa cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK -
氨基酸
32 to 314
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技术指标
Our Abpromise guarantee covers the use of ab64445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 200µl of sterile water.
常规信息
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别名
- Frizzled related protein 1
- FRP
- FRP 1
see all -
功能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
组织特异性
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
序列相似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
结构域
The FZ domain is involved in binding with Wnt ligands. -
细胞定位
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab64445 被引用在 2 文献中.
- Marcos S et al. Secreted frizzled related proteins modulate pathfinding and fasciculation of mouse retina ganglion cell axons by direct and indirect mechanisms. J Neurosci 35:4729-40 (2015). PubMed: 25788689
- Valcz G et al. Myofibroblast-derived SFRP1 as potential inhibitor of colorectal carcinoma field effect. PLoS One 9:e106143 (2014). PubMed: 25405986