Recombinant人SETD7 protein (ab51285)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK

技术指标

Our Abpromise guarantee covers the use of ab51285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和��储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 50mM Tris HCl, 5mM DTT, pH 7.5

常规信息

  • 别名
    • FLJ21193
    • H3 K4 HMTase
    • H3-K4-HMTase SETD7
    • H4 lysine 4 specific
    • Histone H3 K4 methyltransferase
    • Histone H3 lysine 4 specific methyltransferase
    • Histone H3-K4 methyltransferase SETD7
    • Histone H4 K4 methyltransferase
    • Histone lysine N methyltransferase
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7
    • Histone-lysine N-methyltransferase SETD7
    • KIAA1717
    • KMT7
    • Lysine methyltransferase
    • Lysine N-methyltransferase 7
    • OTTHUMP00000164543
    • OTTHUMP00000220049
    • SET 7
    • SET 7/9
    • SET 9
    • SET D7
    • SET domain containing (lysine methyltransferase) 7
    • SET domain containing protein 7
    • SET domain containing protein 8
    • SET domain-containing protein 7
    • SET7
    • SET7/9
    • SET9
    • Setd7
    • SETD7_HUMAN
    see all
  • 功能Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • 组织特异性Widely expressed. Expressed in pancreatic islets.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • 结构域The SET domain is necessary but not sufficient for histone methyltransferase activity.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt

Recombinant Human SETD7 protein 图像

  • ab51285 run on a 10% SDS-PAGE gel with molecular weight markers.
  • Anti-SETD7 antibody - C-terminal (ab71214) at 1/100 dilution + Recombinant Human SETD7 protein (ab51285) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human SETD7 protein (ab51285)参考文献

ab51285 has not yet been referenced specifically in any publications.

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