重组人Serum Albumin蛋白(His tag) (ab217817)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Serum Albumin蛋白(His tag)
参阅全部 Human Serum Albumin 蛋白酶 -
纯度
> 95 % SDS-PAGE.
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFA KTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDL LECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLA KTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGE YKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAE DYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPK EFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDD FAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL -
预测分子量
67 kDa including tags -
氨基酸
25 to 609 -
标签
His tag C-Terminus -
额外的序列信息
The predicted N-terminus is Asp 25. This product is the mature full length protein from aa 25 to 609. The signal peptide and propeptide are not included (NP_000468).
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描述
重组人人Serum Albumin蛋白(His tag)
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab217817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 90% PBS, 10% Trehalose
Lyophilised from 0.22 µm filtered solution. -
复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- alb
- ALBU_HUMAN
- Albumin
see all -
功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
图片
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SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
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The purity of Human Serum Albumin, His Tag (ab217817) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
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Human Serum Albumin Protein, His Tag (ab217817) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Bioactivity SPR: Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab217817 被引用在 1 文献中.
- Srivastava A et al. The Linker Region Promotes Activity and Binding Efficiency of Modular LPMO towards Polymeric Substrate. Microbiol Spectr 10:e0269721 (2022). PubMed: 35080440