Recombinant人Securin protein (ab116478)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionO95997
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMATLIYVDKENGEPGTRVVAKDGLKLGSGP SIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGP LKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDL PEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQ SPSSILSTLDVELPPVCCDIDI
    • 分子量24 kDa including tags
    • 氨基酸1 to 202
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab116478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 80 % SDS-PAGE.
    ab116478 is purified by proprietary chromatographic techniques.
  • 形式Liquid
  • 补充说明although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

常规信息

  • 别名
    • AW555095
    • C87862
    • Cut2
    • EAP 1
    • EAP1
    • ESP1 associated protein 1
    • Esp1-associated protein
    • hPTTG
    • MGC126883
    • MGC138276
    • Pds1
    • Pituitary tumor transforming 1
    • Pituitary tumor transforming protein 1
    • Pituitary tumor-transforming 1, isoform CRA_a
    • Pituitary tumor-transforming 1, isoform CRA_b
    • Pituitary tumor-transforming gene 1
    • Pituitary tumor-transforming gene 1 protein
    • PTTG
    • PTTG 1
    • PTTG1
    • PTTG1 protein
    • PTTG1_HUMAN
    • Pttg3
    • Securin
    • Tumor transforming 1
    • Tumor transforming protein 1
    • Tumor-transforming protein 1
    • TUTR 1
    • TUTR1
    see all
  • 功能Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 组织特异性Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • 序列相似性Belongs to the securin family.
  • 发展阶段Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • 结构域The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • 翻译后修饰Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human Securin protein (ab116478)参考文献

ab116478 has not yet been referenced specifically in any publications.

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