Recombinant人Securin protein (ab116478)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGSSHHHHHHSSGLVPRGSHMATLIYVDKENGEPGTRVVAKDGLKLGSGP SIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGP LKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDL PEEHQIAHLPLSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQ SPSSILSTLDVELPPVCCDIDI
    • 分子量
      24 kDa including tags
    • 氨基酸
      1 to 202
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab116478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 80 % SDS-PAGE.
    ab116478 is purified by proprietary chromatographic techniques.
  • 形式
    Liquid
  • 补充说明
    although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

常规信息

  • 别名
    • AW555095
    • C87862
    • Cut2
    • EAP 1
    • EAP1
    • ESP1 associated protein 1
    • Esp1-associated protein
    • hPTTG
    • MGC126883
    • MGC138276
    • Pds1
    • Pituitary tumor transforming 1
    • Pituitary tumor transforming protein 1
    • Pituitary tumor-transforming 1, isoform CRA_a
    • Pituitary tumor-transforming 1, isoform CRA_b
    • Pituitary tumor-transforming gene 1
    • Pituitary tumor-transforming gene 1 protein
    • PTTG
    • PTTG 1
    • PTTG1
    • PTTG1 protein
    • PTTG1_HUMAN
    • Pttg3
    • Securin
    • Tumor transforming 1
    • Tumor transforming protein 1
    • Tumor-transforming protein 1
    • TUTR 1
    • TUTR1
    see all
  • 功能
    Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • 组织特异性
    Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • 序列相似性
    Belongs to the securin family.
  • 发展阶段
    Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • 结构域
    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • 翻译后修饰
    Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human Securin protein (ab116478)参考文献

ab116478 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116478.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册