Recombinant人SDF1 alpha protein (ab73461)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP48061-2
    • 种属Human
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu
    • 氨基酸22 to 89

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技术指标

Our Abpromise guarantee covers the use of ab73461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.

  • 应用

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    This protein was purified by proprietary chromatographic techniques. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • 补充说明For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    No additives

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • C-X-C motif chemokine 12
    • Chemokine (C-X-C motif) ligand 12
    • cxcl12
    • hIRH
    • hSDF-1
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre-B cell growth-stimulating factor
    • SCYB12
    • SDF 1 alpha
    • SDF-1
    • SDF-1-alpha(3-67)
    • SDF1
    • SDF1_HUMAN
    • SDF1a
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF
    • TPAR1
    see all
  • 功能Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human SDF1 alpha protein (ab73461)参考文献

ab73461 has not yet been referenced specifically in any publications.

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Thank you very much for your call last week and for your patience while I've been in touch with my colleagues regarding your enquiry.
The SDF1 alpha protein, Ab73461, was tested in an activity assay byan outside laboratory, and unfortunately we do...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"