重组人Scavenging Receptor SR-BI蛋白(Fc Chimera) (ab182680)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Scavenging Receptor SR-BI蛋白(Fc Chimera)
参阅全部 Scavenging Receptor SR-BI 蛋白酶 -
生物活性
Measured by its ability to bind recombinant Human ApoAI in a functional ELISA.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
PSLIKQQVLKNVRIDPSSLSFNMWKEIPIPFYLSVYFFDVMNPSEILKGE KPQVRERGPYVYREFRHKSNITFNNNDTVSFLEYRTFQFQPSKSHGSESD YIVMPNILVLGAAVMMENKPMTLKLIMTLAFTTLGERAFMNRTVGEIMWG YKDPLVNLINKYFPGMFPFKDKFGLFAELNNSDSGLFTVFTGVQNISRIH LVDKWNGLSKVDFWHSDQCNMINGTSGQMWPPFMTPESSLEFYSPEACRS MKLMYKESGVFEGIPTYRFVAPKTLFANGSIYPPNEGFCPCLESGIQNVS TCRFSAPLFLSHPHFLNADPVLAEAVTGLHPNQEAHSLFLDIHPVTGIPM NCSVKLQLSLYMKSVAGIGQTGKIEPVVLPLLWFAESGAMEGETLHTFYT QLVLMPKVMHY -
预测分子量
73 kDa including tags -
氨基酸
33 to 443 -
额外的序列信息
Fused with Fc fragment of Human IgG1 at the C-terminus. NP_005496.4. Extracellular domain.
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技术指标
Our Abpromise guarantee covers the use of ab182680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
As a result of glycosylation, DTT-reduced Scavenging Receptor SR-BI protein migrates as 110-115 kDa and non-reduced protein migrates as 220-230 kDa.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.6% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended to reconstitute the lyophilized product in 250 µl sterile deionized water to a final concentration of 200 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
常规信息
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别名
- CD36
- CD36 and LIMPII analogous 1
- CD36 Antigen like 1
see all -
功能
Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the CD36 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab182680 尚未被引用在任何文献中。