Recombinant人SAE1 protein (ab96772)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9UBE0
    • 种属Human
    • 序列MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK
    • 分子量42 kDa including tags
    • 氨基酸1 to 346
    • 标签His tag N-Terminus , T7 tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab96772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF-TOF
  • 纯度> 90 % SDS-PAGE.
    ab96772 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • Activator of SUMO1
    • AOS1
    • HSPC140
    • Sae1
    • SAE1_HUMAN
    • Sentrin/SUMO activating protein AOS1
    • SUA1
    • SUMO 1 activating enzyme E1 N subunit
    • SUMO 1 activating enzyme subunit 1
    • SUMO-activating enzyme subunit 1
    • Ubiquitin like protein SUMO1 activating enzyme
    • Ubiquitin-like 1-activating enzyme E1A
    • UBL E1A
    • UBLE1A
    see all
  • 功能The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • 组织特异性Expression level increases during S phase and drops in G2 phase (at protein level).
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the ubiquitin-activating E1 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human SAE1 protein 图像

  • 15% SDS-PAGE showing ab96772 at approximately 42.2kDa (3µg).

Recombinant Human SAE1 protein (ab96772)参考文献

ab96772 has not yet been referenced specifically in any publications.

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