Recombinant人S100 calcium binding protein A14 (ab101041)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MKHHHHHHASMGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKET LTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGE AAKSVKLERPVRGH
    • 分子量
      13 kDa including tags
    • 氨基酸
      1 to 104
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 形式
    Lyophilised
  • 补充说明
    Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM Tris, pH 7.5

  • 复溶
    Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

常规信息

  • 别名
    • BCMP84
    • Protein S100-A14
    • S100 calcium binding protein A14
    • S100 calcium-binding protein A14
    • S100a14
    • S100A15
    • S10AE_HUMAN
    • S114
    see all
  • 功能
    Modulates P53/TP53 protein levels, and thereby plays a role in the regulation of cell survival and apoptosis. Depending on the context, it can promote cell proliferation or apoptosis. Plays a role in the regulation of cell migration by modulating the levels of MMP2, a matrix protease that is under transcriptional control of P53/TP53. Does not bind calcium.
  • 组织特异性
    Expressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. Low expression in heart and no expression is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the S-100 family.
    Contains 1 EF-hand domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

Recombinant Human S100 calcium binding protein A14 图像

  • 14% SDS-PAGE analysis of ab101041
    Lane 1: Molecular weight markers; 14, 21, 31, 45, 66, 97 kDa
    Lane 2: ab101041 (reduced and heated, 5µg / lane)
    Lane 3: ab101041 (non-reduced and non-heated, 5µg / lane).

Recombinant Human S100 calcium binding protein A14 (ab101041)参考文献

ab101041 has not yet been referenced specifically in any publications.

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