Recombinant人S adenosylhomocysteine hydrolase protein (ab99326)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP23526
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMSDKLPYKVADIGLAAWGRKALDIAENEMP GLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCN IFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILD DGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINV NDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQ ALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCID IILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRL KNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYP VGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHY RY
    • 分子量50 kDa including tags
    • 氨基酸1 to 432
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab99326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab99326 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.40
    Constituents: 20% Glycerol, PBS

常规信息

  • 别名
    • Adenosylhomocysteinase
    • AdoHcyase
    • ahcY
    • S adenosyl L homocysteine hydrolase
    • S adenosylhomocysteine hydrolase
    • S-adenosyl-L-homocysteine hydrolase
    • SAHH
    • SAHH_HUMAN
    see all
  • 功能Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • 通路Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
  • 疾病相关Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
  • 序列相似性Belongs to the adenosylhomocysteinase family.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant Human S adenosylhomocysteine hydrolase protein 图像

  • 15% SDS-PAGE showing ab99326 (3µg).
  • Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml + Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

Recombinant Human S adenosylhomocysteine hydrolase protein (ab99326)参考文献

ab99326 has not yet been referenced specifically in any publications.

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please find below the information we managed to collect about the S adenosylhomocysteine hydrolase protein:

Regrettably, our protein’s biological activity hasn’t been tested yet, and we can&rsq...

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Thank you for contacting Abcam.

We have not tested any of these products for enzymatic activity and so we cannot guarantee that they will have. However, you could take advantage of our testing discount program:

http://www.abcam.com/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"