Recombinant人RRAS2 protein (ab87714)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI RMNVDQAFHE LVRVIRKFQE QECPPSPEPT RKEKDKKGCH C

技术指标

Our Abpromise guarantee covers the use of ab87714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab87714 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, pH 8.0

常规信息

  • 别名
    • C86394
    • Oncogene RRAS2
    • Oncogene TC21
    • Ras like protein TC21
    • Ras related protein R Ras2
    • Ras-like protein TC21
    • Ras-related protein R-Ras2
    • Related RAS viral (r ras) oncogene homolog 2
    • Related RAS viral oncogene homolog 2
    • RRAS 2
    • RRAS2
    • RRAS2_HUMAN
    • TC 21
    • TC21
    • Teratocarcinoma oncogene
    • Teratocarcinoma oncogene TC21
    see all
  • 功能It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.
  • 组织特异性Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.
  • 疾病相关Ovarian cancer
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 翻译后修饰May be post-translationally modified by both palmitoylation and polyisoprenylation.
  • 细胞定位Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
  • Information by UniProt

Recombinant Human RRAS2 protein 图像

  • 15% SDS-PAGE showing ab87714 at approximately 25.2 kDa (3µg).

Recombinant Human RRAS2 protein (ab87714)参考文献

ab87714 has not yet been referenced specifically in any publications.

Product Wall

Application Other
I've used this RRAS2 or TC21 protein for activity assays. TC21 has higher GTPase activity than others members of Ras family. Reading GTP consumption I have recorded good activities (up to 5nM GTP x microg-1 x min-1) without GAP protein. I used also Neurofibromin 1 as GAP and the consumption has increased around 5 times. I also perform coprecipitation assays to pull down activated TC21 with GST-tagged RBD domain of p110delta (in order to measure TC21 activation) and it worked very well with as low as 10ng of TC21. I greatly recommend this product.
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Mr. Pedro Campos

Verified customer

提交于 Dec 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"