Recombinant人ROCK2 protein (ab125828)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      66 kDa including tags
    • 氨基酸
      5 to 554
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab125828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    The specific activity of ab125828 was determined to be 605 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • 形式
    Liquid
  • 补充说明

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.002% PMSF, 0.71% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • coiled-coil-containing protein kinase 2
    • KIAA0619
    • p164 ROCK 2
    • p164 ROCK-2
    • Rho associated coiled coil containing protein kinase 2
    • Rho associated protein kinase 2
    • Rho associated, coiled coil containing protein kinase II
    • Rho kinase 2
    • Rho-associated
    • Rho-associated protein kinase 2
    • ROCK 2
    • Rock II
    • Rock2
    • ROCK2_HUMAN
    • Rock2m
    • ROK alpha
    • ROKalpha
    see all
  • 功能
    Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
  • Information by UniProt

Recombinant human ROCK2 protein 图像

  • SDS-PAGE analysis of ab125828.
  • The specific activity of ab125828 was determined to be 605 nmol/min/mg.

Recombinant human ROCK2 protein (ab125828)参考文献

ab125828 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125828.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册