Recombinant人RhoA protein (ab91068)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab91068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: 0.019% Magnesium chloride, 0.595% HEPES, 0.077% DTT, 0.00088% GDP, 0.232% Sodium chloride

常规信息

  • 别名
    • Aplysia ras related homolog 12
    • ARH12
    • ARHA
    • H 12
    • H12
    • Oncogene RHO H12
    • Ras homolog family member A
    • Ras homolog gene family member A
    • Rho A
    • Rho cDNA clone 12
    • RHO H12
    • RHO12
    • RHOA
    • RHOA_HUMAN
    • RHOH12
    • Small GTP binding protein Rho A
    • Transforming protein Rho A
    • Transforming protein RhoA
    see all
  • 功能
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rho family.
  • 结构域
    The basic-rich region is essential for yopT recognition and cleavage.
  • 翻译后修饰
    Substrate for botulinum ADP-ribosyltransferase.
    Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 细胞定位
    Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt

文献

ab91068 has not yet been referenced specifically in any publications.

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