Recombinant人Retinoid X Receptor alpha protein (ab48737)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MLGLNGVLKV PAHPSGNMAS FTKHICAICG DRSSGKHYGV YSCEGCKGFF KRTVRKDLTY TCRDNKDCLI DKRQRNRCQY CRYQKCLAMG MKREAVQEER QRGKDRNENE VESTSSANE
    • 氨基酸111 to 228

技术指标

Our Abpromise guarantee covers the use of ab48737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    The Retinoid X Receptor alpha protein (111-228aa) was over expressed in E.coli and purified by using conventional column chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 5mM Beta mercaptoethanol, 20mM Tris HCl, pH 7.5

常规信息

  • 别名
    • FLJ00280
    • FLJ00318
    • FLJ16020
    • FLJ16733
    • MGC102720
    • NR2B1
    • Nuclear receptor subfamily 2 group B member 1
    • OTTHUMP00000022510
    • Retinoic acid receptor RXR alpha
    • Retinoic acid receptor RXR-alpha
    • Retinoid X nuclear receptor alpha
    • Retinoid X receptor alpha
    • RXR alpha1
    • Rxra
    • RXRA_HUMAN
    • RXRalpha1
    see all
  • 功能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • 组织特异性Highly expressed in liver, also found in lung, kidney and heart.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • 翻译后修饰Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human Retinoid X Receptor alpha protein 图像

  • 14% SDS-PAGE gel loaded with recombinant human Retinoid X Receptor alpha protein.

Recombinant Human Retinoid X Receptor alpha protein (ab48737)参考文献

ab48737 has not yet been referenced specifically in any publications.

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