概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD

技术指标

Our Abpromise guarantee covers the use of ab63303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.
    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Acetate buffer, pH 4.0

  • 复溶Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.

常规信息

  • 别名
    • FLJ18565
    • Hepatocarcinoma intestine pancreas
    • Hepatointestinal pancreatic protein
    • HIP
    • HIP/PAP
    • Human proislet peptide
    • INGAP
    • Pancreatic beta cell growth factor
    • Pancreatitis associated protein
    • Pancreatitis associated protein 1
    • Pancreatitis-associated protein 1
    • PAP
    • PAP H
    • PAP homologous protein
    • PAP1
    • PBCGF
    • Proliferation inducing protein 34
    • Proliferation inducing protein 42
    • REG III
    • Reg III alpha
    • Reg III-alpha
    • REG-3-alpha
    • REG3
    • Reg3a
    • REG3A_HUMAN
    • Regenerating islet derived 3 alpha
    • Regenerating islet derived protein 3 alpha precursor
    • Regenerating islet-derived protein 3-alpha 15 kDa form
    • Regenerating islet-derived protein III-alpha
    see all
  • 功能Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
  • 组织特异性Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.
  • 序列相似性Contains 1 C-type lectin domain.
  • 结构域The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.
  • 翻译后修饰Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.
  • 细胞定位Secreted. Found in the apical region of pancreatic acinar cells.
  • Information by UniProt

Recombinant Human Reg3a protein 图像

  • 1. MW marker – 14, 21, 31, 45, 66, 97 kDa
    2. reduced and heated sample, 5µg/lane
    3. non-reduced and non-heated sample, 5µg/lane

Recombinant Human Reg3a protein (ab63303)参考文献

This product has been referenced in:
  • Cui W  et al. Overexpression of Reg3alpha increases cell growth and the levels of cyclin D1 and CDK4 in insulinoma cells. Growth Factors 27:195-202 (2009). WB . Read more (PubMed: 19343564) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"