Recombinant人RCC1 protein (ab90625)

概述

  • 产品名称Recombinant人RCC1 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab90625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM HEPES, 2mM DTE (1, 4-Dithioerythritol), pH 7.6

常规信息

  • 别名
    • Cell cycle regulatory protein
    • CHC 1
    • CHC1
    • Chromosome condensation 1
    • Chromosome condensation protein 1
    • Guanine nucleotide releasing protein
    • HERC2
    • Ran GEF
    • RanGEF
    • RCC 1
    • RCC1
    • RCC1 I
    • RCC1_HUMAN
    • Regulator of chromosome condensation
    • Regulator of chromosome condensation 1
    • SHEP1
    • SNHG3 RCC1
    • SNHG3 RCC1 readthrough transcript
    see all
  • 功能Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
  • 序列相似性Contains 7 RCC1 repeats.
  • 翻译后修饰N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di-and trimethylation produce a permanent positive charge on the amino group, which facilitate electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  • 细胞定位Nucleus. Cytoplasm. Becomes dispersed throughout the cytoplasm during mitosis.
  • Information by UniProt

Recombinant Human RCC1 protein 图像

  • All lanes : Anti-RCC1 antibody (ab70749) at 1/2000 dilution

    Lane 1 : Recombinant Human RCC1 protein (ab90625) at 0.01 µg
    Lane 2 : Recombinant Human RCC1 protein (ab90625) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

Recombinant Human RCC1 protein (ab90625)参考文献

ab90625 has not yet been referenced specifically in any publications.

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