Recombinant人Rad51 protein (ab63808)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ06609
    • 种属Human
    • 序列GSHMAMQMQLEAN ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGFHTVE AVAYAPKKEL INIKGISEAK ADKILAEAAK LVPMGFTTAT EFHQRRSEII QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARAFNTDHQ TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD

技术指标

Our Abpromise guarantee covers the use of ab63808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52.

  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度> 90 % SDS-PAGE.
    highly purified by several steps of chromatography
  • 形式Liquid
  • 补充说明

    This protein can be used for: 1) Studies on homologous recombination mechanism and SOS response. 2) Useful in the screening using probe from library by promotion of DNA hybridization. 3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.5mM EDTA, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • BRCA1/BRCA2 containing complex, subunit 5
    • BRCC 5
    • BRCC5
    • DNA repair protein RAD51 homolog 1
    • DNA repair protein rhp51
    • hRAD51
    • HsRAD51
    • HsT16930
    • MRMV2
    • Rad 51
    • RAD51
    • RAD51 homolog
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
    • RAD51 homolog A
    • RAD51 recombinase
    • RAD51, S. cerevisiae, homolog of
    • RAD51_HUMAN
    • RAD51A
    • RECA
    • RecA like protein
    • RecA, E. coli, homolog of
    • Recombination protein A
    see all
  • 功能Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • 组织特异性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • 疾病相关Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • 序列相似性Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • 结构域The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • 翻译后修饰Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt

Recombinant human Rad51 protein 图像

  • SDS-PAGE image show staining with ab63808.

  • All lanes : Anti-Rad51 antibody (ab46981) at 0.5 µg/ml

    Lane 1 : Recombinant human Rad51 protein (ab63808) at 0.1 µg
    Lane 2 : Recombinant human Rad51 protein (ab63808) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

Recombinant human Rad51 protein (ab63808)参考文献

ab63808 has not yet been referenced specifically in any publications.

Product Wall

This recombinant Rad51 is a full length protein (39,966 daltons according to Uniprot has additional peptide) which contains 3 additional amino acids, Gly-Ser-His, at the N-terminus.

Thank you for your enquiry. I can confirm the following regarding ab63808 Rad51 protein: 1). The purity is better > 95% as judged from SDS-PAGE analysis Yes 2). It should possess single strand DNA stimulated ATPase activity. Yes 3). I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"