Recombinant人Rac1 protein (ab89246)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab89246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab89246 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method).
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

常规信息

  • 别名
    • Cell migration inducing gene 5 protein
    • Cell migration-inducing gene 5 protein
    • MGC111543
    • MIG5
    • Migration inducing gene 5
    • Migration inducing protein 5
    • p21 Rac1
    • p21-Rac1
    • Rac 1
    • RAC1
    • RAC1_HUMAN
    • Ras like protein TC25
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
    • Ras-like protein TC25
    • Ras-related C3 botulinum toxin substrate 1
    • Rho family small GTP binding protein Rac1
    • TC 25
    • TC25
    see all
  • 功能Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • 组织特异性Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 结构域The effector region mediates interaction with DEF6.
  • 翻译后修饰AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 细胞定位Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant Human Rac1 protein 图像

  • 15% SDS-PAGE showing ab89246 at approximately 23.6kDa (3µg).

Recombinant Human Rac1 protein (ab89246)参考文献

ab89246 has not yet been referenced specifically in any publications.

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