The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 as determined by the stimulation of protein synthesis in L6 myoblasts is less then 1 ng/ml, corresponding to a specific activity of 1,000,000 units/mg.
The LR3 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. R3 IGF-1 has been produced with the purpose of increasing biological activity.
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Store at -20°C (desiccating conditions).
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute ab125791 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab125791 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Insulin-like growth factor I
Mechano growth factor
R3 IGF1 is an 83 amino acid analog of IGF1 comprising the complete human IGF1 sequence with the substition of an Arg(R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF1 has been produced with the purpose of increasing the biological activity of the IGF peptide.
Insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone.
R3 IGF1 is significantly more potent than IGF1. The enhanced potency is due to the decreased binding of Long R3 IGF1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF’s.
Recombinant human R3 IGF1 protein (ab125791)参考文献
has not yet been referenced specifically in any publications.