重组人PSIP1/LEDGF蛋白(ab82128)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, GSA
描述
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产品名称
重组人PSIP1/LEDGF蛋白
参阅全部 PSIP1/LEDGF 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Purified by affinity chromatography in combination with FPLC chromatography. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
额外的序列信息
The amino acid sequence for ab82128 corresponds to isoform 1 of the protein, which is also known as p75 and PSIP1. Accession number NM_033222.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab82128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Gel Supershift Assays
EMSA
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形式
Liquid -
补充说明
This product was previously labelled as PSIP1
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- CLL associated antigen KW 7
- CLL-associated antigen KW-7
- Dense fine speckles 70 kDa protein
see all -
功能
Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. -
组织特异性
Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. -
疾病相关
Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
序列相似性
Belongs to the HDGF family.
Contains 1 PWWP domain. -
结构域
Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain). -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Remains chromatin-associated throughout the cell cycle. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab82128 尚未被引用在任何文献中。