Recombinant人Protein Kinase A regulatory subunit I alpha (ab68967)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab68967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Unit Definition: One unit is defined as the amount of Protein Kinase A regulatory subunit I alpha, required to incorporate 1nmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30°C.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    This protein was purified by proprietary chromatographic techniques.
  • 形式Liquid
  • 补充说明This protein is cAMP free. Assay Conditions: Roskoski-Assay The assay will be performed in a mixture containing 50mM MOPS (pH7.0), 10mM MgCI2, 0.25 mg/ml bovine serum albumin, 100 IJM Kemptide (peptide substrate), 100 IJM unlabeled ATP mixed with [y_32p] ATP (500-1000 cpm/pmol) and Ca subunit in a final volume of 50 IJI. Reaction is started by addition of the Ca subunit and can be stopped after a 5 minutes incubation at 30°C by spotting the reaction mix onto Whatman P-81 filters and soaking the filters four times in 75mM phosphoric acid (10 ml per sample) for at least 5 minutes. After four washing steps rinse filters with ethanol, dry and count. For the detection of phosphorylation in substrate proteins the phosphotransferase reaction can alternatively be stopped by taking aliquots of the mixture and adding SDS sample buffer. The phosphorylation status of the substrate proteins can subsequently be analysed using SDS PAGE and autoradiography. Unit Definition: One unit is defined as the amount of Protein Kinase A regulatory subunit I alpha, required to incorporate 1nmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30°C.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • ACRDYS1
    • ADOHR
    • cAMP dependent protein kinase regulatory subunit alpha 1
    • cAMP dependent protein kinase regulatory subunit RIalpha
    • cAMP dependent protein kinase type I alpha regulatory chain
    • cAMP dependent protein kinase type I alpha regulatory subunit
    • cAMP-dependent protein kinase type I-alpha regulatory subunit
    • CAR
    • Carney complex type 1
    • CNC
    • CNC 1
    • CNC1
    • DKFZp779L0468
    • KAP0_HUMAN
    • MGC17251
    • PKA RIA
    • PKR 1
    • PKR1
    • PPNAD 1
    • PPNAD1
    • PRKAR 1
    • PRKAR1
    • PRKAR1A
    • PRKAR1A/RARA FUSION GENE
    • Protein kinase A type 1a regulatory subunit
    • Protein kinase cAMP dependent regulatory type I alpha
    • PTC2 CHIMERIC ONCOGENE, INCLUDED
    • Tissue specific extinguisher 1
    • Tissue-specific extinguisher 1
    • TSE 1
    • TSE1
    see all
  • 组织特异性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • 疾病相关Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
    Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
    Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
  • 序列相似性Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • 翻译后修饰The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
  • Information by UniProt

Recombinant human Protein Kinase A regulatory subunit I alpha (ab68967)参考文献

ab68967 has not yet been referenced specifically in any publications.

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