Recombinant人Prostaglandin D Synthase (Lipocalin) protein (ab63850)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MRGSHHHHHH GMASAPEAQV SVQPNFQQDK FLGRWFSAGL ASNSSWLREK KAALSMCKSV VAPATDGGLN LTSTFLRKNQ CETRTMLLQP AGSLGSYSYR SPHWGSTYSV SVVETDYDQY ALLYSQGSKG PGEDFRMATL YSRTQTPRAE LKEKFTAFCK AQGFTEDTIV FLPQTDKCMT EQ
    • 氨基酸23 to 190

技术指标

Our Abpromise guarantee covers the use of ab63850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Sodium chloride, 20mM Tris buffer, pH 7.5

  • 复溶Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

常规信息

  • 别名
    • Beta trace protein
    • Beta-trace protein
    • Cerebrin 28
    • Cerebrin-28
    • glutathione independent PGD synthase
    • Glutathione independent PGD synthetase
    • Glutathione-independent PGD synthase
    • L PGDS
    • Lipocalin type prostaglandin D synthase
    • Lipocalin-type prostaglandin-D synthase
    • LPGDS
    • PDS
    • PGD2
    • PGD2 synthase
    • PGDS
    • PGDS2
    • prostaglandin D synthase
    • Prostaglandin D2 synthase
    • prostaglandin D2 synthase (21 kDa, brain)
    • prostaglandin D2 synthase (brain)
    • prostaglandin D2 synthase 21kDa (brain)
    • prostaglandin H2 D isomerase
    • Prostaglandin-D2 synthase
    • Prostaglandin-H2 D-isomerase
    • PTGDS
    • PTGDS_HUMAN
    • RP23-47P18.11-006
    see all
  • 功能Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
  • 组织特异性Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 发展阶段Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks.
  • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 翻译后修饰Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present.
  • 细胞定位Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
  • Information by UniProt

Recombinant Human Prostaglandin D Synthase (Lipocalin) protein 图像

  • 12% SDS-PAGE separation of ab63850. Molecular Weight: ~20.3 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

Recombinant Human Prostaglandin D Synthase (Lipocalin) protein (ab63850)参考文献

ab63850 has not yet been referenced specifically in any publications.

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