重组人Prorenin蛋白(Active) (ab93266)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
描述
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产品名称
重组人Prorenin蛋白(Active)
参阅全部 Prorenin 蛋白酶 -
生物活性
Fully activatable to renin by catalytic amounts of trypsin.
ab93266 is > 95% active.
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纯度
> 95 % SDS-PAGE.
ab93266 is purified by affinity chromatography. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
44 kDa
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技术指标
Our Abpromise guarantee covers the use of ab93266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
Extinction coefficient: 1.1
UV Absorbance (280 nm)=0.99
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.79% Tris HCl, 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Angiotensin forming enzyme
- Angiotensinogenase
- FLJ10761
see all -
功能
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
疾病相关
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
序列相似性
Belongs to the peptidase A1 family. -
细胞定位
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab93266 被引用在 3 文献中.
- Ooi K et al. Sigma-1 Receptor Activation Suppresses Microglia M1 Polarization via Regulating Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Functions in Stress-Induced Hypertension Rats. Mol Neurobiol 58:6625-6646 (2021). PubMed: 34601668
- Hu L et al. Microglia-Derived NLRP3 Activation Mediates the Pressor Effect of Prorenin in the Rostral Ventrolateral Medulla of Stress-Induced Hypertensive Rats. Neurosci Bull 36:475-492 (2020). PubMed: 32242284
- Hu L et al. Melatonin decreases M1 polarization via attenuating mitochondrial oxidative damage depending on UCP2 pathway in prorenin-treated microglia. PLoS One 14:e0212138 (2019). PubMed: 30742657