Recombinant人Prolyl Endopeptidase protein (ab80376)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      MHHHHHHLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVE AQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGL QNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLS ASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQD GKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDG RYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVT NEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACV RSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEI FYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPS KDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRH MGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTS PKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHA WTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRV VPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVS DMFAFIARCLNVDWIP
    • 分子量
      82 kDa
    • 氨基酸
      2 to 710

技术指标

Our Abpromise guarantee covers the use of ab80376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific Activity: ≥ 12 pmol/min/µg. Assay condition: 50 mM Tris (pH 7.5), 100 µg BSA, 20 µM substrate and 500 ng Prolyl Endopeptidase protein at room temperature for 30 min.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • dJ355L5.1 (prolyl endopeptidase)
    • HGNC:9358
    • MGC16060
    • OTTHUMP00000040498
    • PE
    • PEP
    • Post proline cleaving enzyme
    • Post-proline cleaving enzyme
    • PPCE_HUMAN
    • Prep
    • Prolyl endopeptidase
    • Prolyl oligopeptidase
    see all
  • 功能
    Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
  • 序列相似性
    Belongs to the peptidase S9A family.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt

图片

  • Lane 1: ab80376 on 10% SDS-PAGE, Coomassie staining, 4µg.
    Lane 2: Protein marker.

文献

ab80376 has not yet been referenced specifically in any publications.

客户评价及客户问答



Assay condition: 50 mM Tris (pH 7.5), 0.1% BSA, 20 uM substrate (A-P-AMC), and 500 ng ab80376 at room temperature for 30 min.

I hope this is helpful. Please feel free to contact me again with any further questions.


We don’t think POP can cleave BSA. See below for the answer. Some information taken from references.

In humans prolyl endopeptidase is encoded by the PREP gene.[5][6] The protein encoded by this gene is a cytosolic prolyl endopeptidase ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册