Recombinant人Prolyl Endopeptidase protein (ab80376)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MHHHHHHLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVE AQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGL QNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLS ASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQD GKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDG RYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVT NEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACV RSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEI FYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPS KDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRH MGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTS PKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHA WTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRV VPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVS DMFAFIARCLNVDWIP
    • 分子量82 kDa
    • 氨基酸2 to 710

技术指标

Our Abpromise guarantee covers the use of ab80376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific Activity: ≥ 12 pmol/min/µg. Assay condition: 50 mM Tris (pH 7.5), 100 µg BSA, 20 µM substrate and 500 ng Prolyl Endopeptidase protein at room temperature for 30 min.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • dJ355L5.1 (prolyl endopeptidase)
    • HGNC:9358
    • MGC16060
    • OTTHUMP00000040498
    • PE
    • PEP
    • Post proline cleaving enzyme
    • Post-proline cleaving enzyme
    • PPCE_HUMAN
    • Prep
    • Prolyl endopeptidase
    • Prolyl oligopeptidase
    see all
  • 功能Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
  • 序列相似性Belongs to the peptidase S9A family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human Prolyl Endopeptidase protein 图像

  • Lane 1: ab80376 on 10% SDS-PAGE, Coomassie staining, 4µg.
    Lane 2: Protein marker.

Recombinant human Prolyl Endopeptidase protein (ab80376)参考文献

ab80376 has not yet been referenced specifically in any publications.

Product Wall



Assay condition: 50 mM Tris (pH 7.5), 0.1% BSA, 20 uM substrate (A-P-AMC), and 500 ng ab80376 at room temperature for 30 min.

I hope this is helpful. Please feel free to contact me again with any further questions.


We don’t think POP can cleave BSA. See below for the answer. Some information taken from references.

In humans prolyl endopeptidase is encoded by the PREP gene.[5][6] The protein encoded by this gene is a cytosolic prolyl endopeptidase ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"