Recombinant人Procathepsin K protein (ab123765)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP43235
    • 种属Human
    • 分子量35 kDa
    • 氨基酸19 to 329

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技术指标

Our Abpromise guarantee covers the use of ab123765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性SPECFIC ACTIVITY: After auto-catalytical activation at pH 4.0 the specific activity is >1000 mU/mg (international unit 1µmole/min/mg). ACTIVATION: Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT. Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine’s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups. This reversible reaction arrests the autoproteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Tris, 2.92% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Cathepsin K
    • Cathepsin O
    • Cathepsin O1
    • Cathepsin O2
    • Cathepsin X
    • CATK_HUMAN
    • Ctsk
    • CTSO
    • CTSO2
    • PKND
    • PYCD
    see all
  • 功能Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • 组织特异性Predominantly expressed in osteclasts (bones).
  • 疾病相关Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • 序列相似性Belongs to the peptidase C1 family.
  • 细胞定位Lysosome.
  • Information by UniProt

Recombinant human Procathepsin K protein 图像

  • SDS-PAGE showing ab123765.

Recombinant human Procathepsin K protein (ab123765)参考文献

ab123765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"