Recombinant人PPP1A protein (ab113150)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP62136
    • 种属Human
    • 分子量40 kDa including tags
    • 氨基酸1 to 330
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab113150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性>3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 °C, pH 7.5, using 10 mM of substrate.
  • 应用

    SDS-PAGE

    Mass Spectrometry

    Functional Studies

  • 质谱法
    MALDI-TOF
  • 纯度> 80 % SDS-PAGE.
    ab113150 was purified using conventional chromatography techniques.
  • 形式Liquid
  • 补充说明


  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.02% DTT, 0.79% Tris HCl, 50% Glycerol, 0.88% Sodium chloride, 0.01% Manganese chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit
    • Catalytic subunit
    • EC 3.1.3.16
    • MGC15877
    • MGC1674
    • PP 1A
    • PP-1A
    • PP1A
    • PP1A_HUMAN
    • PP1alpha
    • PP2C ALPHA
    • PP2CA
    • Ppp1ca
    • Protein Phosphatase 2C Alpha Isoform
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
    see all
  • 功能Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • 序列相似性Belongs to the PPP phosphatase family. PP-1 subfamily.
  • 细胞定位Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt

Recombinant human PPP1A protein 图像

  • 15% SDS-PAGE showing ab113150 (3 µg) at approximately 39.7 kDa.

Recombinant human PPP1A protein (ab113150)参考文献

ab113150 has not yet been referenced specifically in any publications.

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