Recombinant人PPM1G protein (ab109936)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionO15355
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMGAYLSQPNTVKCSGDGVGAPRLPLPYGFS AMQGWRVSMEDAHNCIPELDSETAMFSVYDGHGGEEVALYCAKYLPDIIK DQKAYKEGKLQKALEDAFLAIDAKLTTEEVIKELAQIAGRPTEDEDEKEK VADEDDVDNEEAALLHEEATMTIEELLTRYGQNCHKGPPHSKSGGGTGEE PGSQGLNGEAGPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEP GIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEE EDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTT AVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNA GGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLT DDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELL DQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEG AEENGNSDKKKKAKRD
    • 分子量61 kDa including tags
    • 氨基酸1 to 546
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10mM of substrate.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 85 % SDS-PAGE.
    ab109936 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT, 0.002% PMSF

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • MGC1675
    • MGC2870
    • OTTHUMP00000123426
    • PP2C
    • PP2C gamma
    • PP2C-gamma
    • PP2CG
    • PP2Cgamma
    • PPM1G
    • PPM1G_HUMAN
    • PPP2CG
    • Protein phosphatase 1C
    • Protein phosphatase 1G
    • Protein phosphatase 2 catalytic subunit gamma isoform
    • Protein phosphatase 2C gamma isoform
    • Protein phosphatase 2C isoform gamma
    • Protein phosphatase magnesium dependent 1 gamma
    • Protein phosphatase magnesium-dependent 1 gamma
    • Protein phosphatase Mg2+/Mn2+ dependent 1G
    see all
  • 组织特异性Widely expressed. Most abundant in testis, skeletal muscle, and heart.
  • 序列相似性Belongs to the PP2C family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human PPM1G protein 图像

  • 15% SDS-PAGE showing ab109936 (3µg).

Recombinant human PPM1G protein (ab109936)参考文献

ab109936 has not yet been referenced specifically in any publications.

Product Wall

Here is the assay protocolused to assess protein activityfor ab109936:

1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
2. Pre-warm react...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"