Recombinant人PPM1A protein (ab51205)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
    • 氨基酸1 to 382

技术指标

Our Abpromise guarantee covers the use of ab51205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: > 8,000units/mg of PPM1A. Unit Definition - one unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate.
  • 应用

    SDS-PAGE

    Functional Studies

  • 内毒素水平< 1.000 Eu/µg
  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab51205 was purified by conventional column chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 50mM Sodium chloride, 2mM DTT, 10mM Tris HCl, 1mM Manganese chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Protein phosphatase 2C isoform alpha
    • EC 3.1.3.16
    • FLJ42306
    • IA
    • MGC9201
    • Mpp alpha
    • PP2C alpha
    • PP2C-alpha
    • PP2CA
    • PP2Calpha
    • PPM 1A
    • PPM1A
    • PPM1A_HUMAN
    • PPPM1A
    • Protein phosphatase 1A
    • Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform
    • Protein phosphatase 1A magnesium dependent alpha
    • Protein phosphatase 2C alpha
    • Protein phosphatase 2C alpha isoform
    • Protein phosphatase 2C isoform alpha
    • Protein phosphatase IA
    • Protein phosphatase, Mg2+/Mn2+ dependent, 1A
    see all
  • 功能Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
  • 序列相似性Belongs to the PP2C family.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant human PPM1A protein 图像

  • 10% SDS-PAGE gel loaded with ab51205, PP2C alpha protein (His tag).
    Predicted molecular weight 46.6 kDa.

Recombinant human PPM1A protein (ab51205)参考文献

ab51205 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"