Recombinant人PPIL3 protein (ab113119)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9H2H8
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMSVTLHTDVGDIKIEVFCERTPKTCENFLA LCASNYYNGCIFHRNIKGFMVQTGDPTGTGRGGNSIWGKKFEDEYSEYLK HNVRGVVSMANNGPNTNGSQFFITYGKQPHLDMKYTVFGKVIDGLETLDE LEKLPVNEKTYRPLNDVHIKDITIHANPFAQ
    • 分子量20 kDa including tags
    • 氨基酸1 to 161
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab113119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant PPIL3 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • 应用

    Mass Spectrometry

    Functional Studies

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab113119 was purified using conventional chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride, 20% Glycerol, 0.02% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 2310076N22Rik
    • 2510026K04Rik
    • Ccyclophilin like protein
    • Cyclophilin J
    • Cyclophilin like 3
    • Cyclophilin like protein 3
    • Cyclophilin like protein PPIL3
    • Cyclophilin-like protein PPIL3
    • Cyp10l
    • CyPJ
    • MGC105285
    • Peptidyl prolyl cis trans isomerase like 3
    • Peptidyl-prolyl cis-trans isomerase-like 3
    • Peptidylprolyl cis trans isomerase like protein 3
    • Peptidylprolyl isomerase (cyclophilin) like 3
    • Peptidylprolyl isomerase like 3
    • PPIase
    • PPIase like protein 3
    • Ppil3
    • PPIL3_HUMAN
    • Rotamase
    • Rotamase PPIL3
    see all
  • 功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
  • 组织特异性Ubiquitous. Detected at low levels.
  • 序列相似性Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • Information by UniProt

Recombinant human PPIL3 protein 图像

  • 15% SDS-PAGE analysis of ab113119 (3µg)

Recombinant human PPIL3 protein (ab113119)参考文献

ab113119 has not yet been referenced specifically in any publications.

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