Recombinant人PPIL1 protein (ab79247)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNG TKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAM ANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQ DRPVDDVKIIKAYPSGLEHHHHHH

技术指标

Our Abpromise guarantee covers the use of ab79247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant PPIL1 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1deg;C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    ab79247 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CGI 124
    • CGI-124
    • CGI124
    • Cyclophilin related gene 1
    • cyclophilin-related gene 1
    • cyclophilinrelated gene 1
    • CYPL1
    • hCyPX
    • MGC678
    • peptidyl-prolyl cis-trans isomerase
    • Peptidyl-prolyl cis-trans isomerase-like 1
    • Peptidylprolyl isomerase (cyclophilin) like 1
    • peptidylprolyl isomerase (cyclophilin)-like 1
    • peptidylprolyl isomerase (cyclophilin)like 1
    • peptidylprolyl isomerase (cyclophilin)like1
    • peptidylprolyl isomerase like 1
    • PPIase
    • PPIL1
    • PPIL1_HUMAN
    • rotamase
    • Rotamase PPIL1
    see all
  • 功能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
  • 组织特异性Ubiquitous, with the most abundant expression in heart and skeletal muscle.
  • 序列相似性Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • Information by UniProt

Recombinant human PPIL1 protein 图像

  • 15% SDS-PAGE showing ab79247 at approximately 19kDa (3µg).
  • All lanes : Anti-PPIL1 antibody (ab57195) at 1 µg/ml

    Lane 1 : Recombinant human PPIL1 protein (ab79247) at 0.1 µg
    Lane 2 : Recombinant human PPIL1 protein (ab79247) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant human PPIL1 protein (ab79247)参考文献

ab79247 has not yet been referenced specifically in any publications.

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