Recombinant人PP2A beta protein (ab114425)

概述

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRC PVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLL VALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLF DYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLW SDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNW CHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHV TRRTPDYFL
    • 分子量
      60 kDa including tags
    • 氨基酸
      1 to 309

技术指标

Our Abpromise guarantee covers the use of ab114425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    ELISA

    Western blot

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • PP2A beta
    • PP2A-beta
    • PP2AB_HUMAN
    • PP2Abeta
    • PP2CB
    • Ppp2cb
    • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
    • Protein phosphatase 2 catalytic subunit beta isozyme
    • Protein phosphatase 2, catalytic subunit, beta isoform
    • Protein phosphatase 2A catalytic subunit beta isoform
    • Protein phosphatase type 2A catalytic subunit
    • Serine/threonine protein phosphatase 2A catalytic subunit beta
    • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
    see all
  • 功能
    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • 翻译后修饰
    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • 细胞定位
    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
  • Information by UniProt

图片

  • ab114425 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

文献

ab114425 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab114425.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册