Recombinant人PLK4 protein (ab125558)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionO00444
    • 种属Human
    • 分子量140 kDa including tags
    • 氨基酸1 to 836

技术指标

Our Abpromise guarantee covers the use of ab125558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab125558 is 5.6 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 70 % Densitometry.
    ab125558 is >70% pure.
  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • PLK 4
    • PLK-4
    • PLK4
    • PLK4_HUMAN
    • Polo like Kinase 4
    • Polo-like kinase 4
    • Protein serine/threonine kinase
    • SAK
    • Serine/threonine kinase
    • Serine/Threonine Kinase 18
    • Serine/threonine protein kinase 18
    • Serine/threonine protein kinase PLK4
    • Serine/threonine protein kinase Sak
    • Serine/threonine-protein kinase 18
    • Serine/threonine-protein kinase PLK4
    • Serine/threonine-protein kinase SAK
    • Snk Akin Kinase
    • STK 18
    • STK18
    see all
  • 功能Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt

Recombinant human PLK4 protein 图像

  • The specific activity of ab125558 was determined to be 5.6 nmol/min/mg.
  • SDS PAGE analysis of ab125558 (PLK4 protein).

Recombinant human PLK4 protein (ab125558)参考文献

ab125558 has not yet been referenced specifically in any publications.

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