The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The PLFG-2 is fully biologically active when compared with standards. Recombinant human PIGF-2 can bind to immobilized rh-sFlt-1 (100 ng/well) with linear range at 0.3-10 ng/ml.
The lyophilized protein is best stored desiccated below 0oC
Following reconstitution, the solution can be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Prevent freeze/thaw cycles.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in sterile 20 mM acetic acid to a concentration of 0.1-1.0 µg/microl.
Placental growth factor
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF, isolated from a human placental cDNA library, has 53% amino acid sequence homology in the PDGF-region of VEGF. As a result of alternative splicing, three human isoform precursors (PlGF1, PlGF2, and PlGF3) have been described. In mouse tissues, there is only one mRNA encoding the equivalent of human PlGF2.3 Mouse PlGF2 encodes a 158 amino acid precursor protein that shares a 65% homology with human PlGF2. The gene for PlGF is organized into seven exons and is mapped to chromosome 14. Placenta growth factor will potentiate VEGF that is present at low concentrations in vitro and in vivo. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
Secreted (by similarity).
has not yet been referenced specifically in any publications.