The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Purity: > 95% by SDS-PAGE and visualised by silver stain.
Endotoxin level: < 0.1 ng per ug of PIGF1.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The lyophilised PlGF1 is supplied in lyophilized form with carrier-protein (HSA) and can be reconstituted with 0.1 M acetic acid or PBS. This solution can be diluted into other buffered solutions or stored frozen for future use.
Placenta growth factor
Placental growth factor is found as three isoforms, PLGF1, PLGF2 and PLGF3 created by alternative splicing. They are secreted factors although PLGF2 remains attached to the cell unless releasd by heparin. PLGF1 is active in angiogenesis, and endothelial cell growth, stimulating proliferation and migration. It binds to receptor VEGFR-1/FLT1.
Recombinant Human PLGF1 protein (ab54336)参考文献
has not yet been referenced specifically in any publications.