Recombinant人PKMYT1 protein (ab125565)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      93 kDa including tags
    • 氨基酸
      1 to 499

技术指标

Our Abpromise guarantee covers the use of ab125565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 80 % Densitometry.
    Purity was determined to be >80% by densitometry.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

常规信息

  • 别名
    • cdc2 inhibitory kinase
    • DKFZp547K1610
    • FLJ20093
    • Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase
    • Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
    • MYT1
    • Myt1 kinase
    • PKMYT 1
    • Pkmyt1
    • PMYT1_HUMAN
    • PPP1R126
    • Protein kinase membrane associated tyrosine/threonine 1
    • Protein kinase Myt1
    • Protein phosphatase 1 regulatory subunit 126
    see all
  • 功能
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
  • 序列相似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
  • 翻译后修饰
    Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
  • 细胞定位
    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt

Recombinant Human PKMYT1 protein 图像

  • SDS-PAGE analysis of ab125565.

Recombinant Human PKMYT1 protein (ab125565)参考文献

ab125565 has not yet been referenced specifically in any publications.

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