Recombinant人PKM2 protein (ab89364)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 序列
      MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab89364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific activity: > 5.0 unit/mg. One unit will form 1.0 µmol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.
  • 应用

    Functional Studies

    Western blot

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab89364 is purified using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CTHBP
    • Cytosolic thyroid hormone binding protein
    • Cytosolic thyroid hormone-binding protein
    • KPYM_HUMAN
    • MGC3932
    • OIP 3
    • OIP-3
    • OIP3
    • OPA interacting protein 3
    • Opa-interacting protein 3
    • p58
    • PK muscle type
    • PK, muscle type
    • PK2
    • PK3
    • PKM
    • PKM2
    • pykm
    • Pyruvate kinase 2/3
    • Pyruvate kinase 3
    • Pyruvate kinase isozymes M1/M2
    • Pyruvate kinase muscle
    • Pyruvate kinase muscle isozyme
    • pyruvate kinase PKM
    • Pyruvate kinase, muscle 2
    • TCB
    • THBP1
    • Thyroid hormone binding protein 1
    • Thyroid hormone binding protein cytosolic
    • Thyroid hormone-binding protein 1
    • Tumor M2 PK
    • Tumor M2-PK
    see all
  • 功能
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 组织特异性
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • 通路
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 序列相似性
    Belongs to the pyruvate kinase family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 细胞定位
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt

图片

  • 15% SDS-PAGE showing ab89364 at approximately 60.1kDa (3µg).

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

文献

ab89364 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Other

Review text: The protein is of a excellent quality and it is working for functional assays.
Sample: Human Purified protein

Primary antibody (in addition to 'PKM2 protein (Active)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
Abcam response: We would like to thank you for taking thing time to submit an Abreview for this product. With publication of this review you have gained Abpoints which may be redeemed for future products or gift cards! If you submit an image which we publish with the Abreview, you will get an additional 100 Abpoints!

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提交于 Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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