Recombinant人PKLR protein (ab100984)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP30613
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMLTQELGTAFFQQQQLPAAMADTFLEHLCL LDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHE YHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEV ELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLIS LVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFG VEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDE ILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLE SMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIA REAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLT TTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIW ADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
    • 分子量59 kDa including tags
    • 氨基酸47 to 574
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab100984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.
    Activity Assay:
    1. Prepare a 1.45 ml reaction mixture into a suitable container.
      - Reaction mixture: 100mM Tris-HCl pH 7.5, 7.6 mM ADP, 15 mM MgCl2, 74 mM KCl, 0.2 mM Beta-NADH, 5.2 mM PEP, 0.025 units Recombinant LDHA protein (ab93699)
    2. Add 50ul of recombinant PKLR protein solution with various concentrations (0.5ug, 1ug)
    3. Read the decrease in A340nm in kinetic mode for 10 minutes.
  • 应用

    Functional Studies

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab100984 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EC 2.7.1.40
    • KPYR_HUMAN
    • L-PK
    • Pk-1
    • PK1
    • PKL
    • Pklg
    • Pklr
    • PKR
    • PKRL
    • Pyruvate kinase 1
    • Pyruvate kinase isozymes R/L
    • Pyruvate kinase liver and blood cell
    • Pyruvate kinase liver and RBC
    • Pyruvate kinase liver and red blood cell
    • Pyruvate kinase liver type
    • Pyruvate kinase type L
    • Pyruvate kinase, red cell type
    • R type/L type pyruvate kinase
    • R-PK
    • R-type/L-type pyruvate kinase
    • Red cell/liver pyruvate kinase
    • RPK
    see all
  • 功能Plays a key role in glycolysis.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 疾病相关Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • 序列相似性Belongs to the pyruvate kinase family.
  • Information by UniProt

Recombinant human PKLR protein 图像

  • 15% SDS-PAGE analysis of 3µg ab100984.

Recombinant human PKLR protein (ab100984)参考文献

ab100984 has not yet been referenced specifically in any publications.

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