Recombinant人PKC zeta protein (ab60848)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human
    • 标签
      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab60848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • 形式
    Liquid
  • 补充说明

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 14-3-3-zetaisoform
    • AI098070
    • aPKCzeta
    • C80388
    • EC 2.7.11.13
    • KPCZ_HUMAN
    • nPKC zeta
    • nPKC-zeta
    • OTTHUMP00000001368
    • OTTHUMP00000044160
    • PKC 2
    • PKC ZETA
    • PKC2
    • Pkcz
    • PKCZETA
    • PKM-zeta, included
    • PRKCZ
    • Protein kinase C zeta
    • Protein kinase C zeta form
    • Protein kinase C zeta type
    • r14-3-3
    • R74924
    • zetaPKC
    see all
  • 功能
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • 组织特异性
    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 结构域
    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • 翻译后修饰
    CDH5 is required for its phosphorylation at Thr-410.
  • 细胞定位
    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt

图片

  • Sample Kinase Activity Plot.
  • ab60848 on SDS-PAGE, MW ~93 kDa.

文献

ab60848 has not yet been referenced specifically in any publications.

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