Recombinant人PKC iota protein (ab60850)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab60850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • aPKC lambda/iota
    • aPKC-lambda/iota
    • Atypical protein kinase C lambda/iota
    • Atypical protein kinase C-lambda/iota
    • DXS1179E
    • KPCI_HUMAN
    • MGC26534
    • nPKC iota
    • nPKC-iota
    • nPKCiota
    • PKC lambda
    • PKC lambda/iota
    • PKCI
    • PKCiota
    • PKClambda
    • PRKC iota
    • PRKC lambda/iota
    • PRKC-lambda/iota
    • PRKCI
    • Protein kinase C iota
    • Protein kinase C iota type
    see all
  • 功能Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
  • 组织特异性Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • 结构域The OPR domain mediates interaction with SQSTM1.
    The C1 domain does not bind diacylglycerol (DAG).
  • 翻译后修饰On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
  • 细胞定位Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
  • Information by UniProt

Recombinant human PKC iota protein 图像

  • Sample Kinase Activity Plot.
  • ab60850 on SDS-PAGE, MW ~98 kDa.
  • Anti-PKC iota antibody (ab5282) at 1/500 dilution + Recombinant human PKC iota protein (ab60850) at 0.1 µg

    Secondary
    Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Recombinant human PKC iota protein (ab60850)参考文献

ab60850 has not yet been referenced specifically in any publications.

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