Recombinant人PKC epsilon protein (ab73538)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 氨基酸2 to 737

技术指标

Our Abpromise guarantee covers the use of ab73538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 90 % SDS-PAGE.
    ab73538 is purified by proprietary chromatographic techniques and filter sterilized.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.03% Brij 35, 0.1M Sodium chloride, 20mM Tris, 1mM Benzamidine, 1mM DTT, 0.2mM PMSF, 0.1mM EDTA, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • KPCE_HUMAN
    • MGC125656
    • MGC125657
    • nPKC epsilon
    • nPKC-epsilon
    • PKCE
    • Pkcea
    • PRKCE
    • Protein kinase C epsilon
    • Protein kinase C epsilon type
    see all
  • 功能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻译后修饰Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt

Recombinant human PKC epsilon protein (ab73538)参考文献

ab73538 has not yet been referenced specifically in any publications.

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