Recombinant人PKC delta protein (ab60844)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab60844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific Activity: 302 nmol/min/mg.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 形式
    Liquid
  • 补充说明


    Phosphatidylinositol is the most potent activator of PKC delta.

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CVID9
    • D14Ertd420e
    • Kinase PKC delta
    • KPCD
    • KPCD_HUMAN
    • MAY 1
    • MAY1
    • MGC49908
    • nPKC delta
    • nPKC-delta
    • PCKd
    • PKC d
    • PKC delta
    • PKCD
    • PKCdelta
    • PRKC D
    • PRKC delta
    • Prkcd
    • Protein Kinase C delta
    • Protein kinase C delta type
    • Protein kinase C delta VIII
    • Protein Kinase Cdelta
    • Tyrosine protein kinase PRKCD
    see all
  • 功能
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 结构域
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • 翻译后修饰
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • 细胞定位
    Cytoplasm. Membrane.
  • Information by UniProt

图片

  • Sample Kinase Activity Plot.
  • ab60844 on SDS-PAGE, MW ~104 kDa.

文献

ab60844 has not yet been referenced specifically in any publications.

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