Recombinant人PKC alpha protein (ab55672)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab55672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity 3333nmol/min/mg.
  • 应用

    Functional Studies

    Western blot

    SDS-PAGE

    Other

  • 形式Liquid
  • 补充说明

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • AAG6
    • Aging associated gene 6
    • aPKC
    • KPCA_HUMAN
    • PKC alpha
    • PKC-A
    • PKC-alpha
    • PKCA
    • PRKACA
    • PRKCA
    • Protein Kinase C alpha
    • Protein kinase C alpha type
    see all
  • 功能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

Recombinant human PKC alpha protein 图像

  • SDS Page analysis of ab55672
  • Anti-PKC alpha antibody (ab66181) at 1 µg/ml + Recombinant human PKC alpha protein (ab55672) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa


    Exposure time : 10 seconds

Recombinant human PKC alpha protein (ab55672)参考文献

ab55672 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab55672.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"