重组人PKA beta (catalytic subunit)蛋白(ab60837)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人PKA beta (catalytic subunit)蛋白
参阅全部 PKA beta (catalytic subunit) 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
65 kDa including tags -
标签
GST tag N-Terminus
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相关产品
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Affibody® Molecule
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
- cAMP-dependent protein kinase catalytic subunit beta
- KAPCB_HUMAN
see all -
功能
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. -
组织特异性
Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
翻译后修饰
Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. -
细胞定位
Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60837 尚未被引用在任何文献中。