Recombinant人PIM1 protein (ab60835)

概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属
      Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab60835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • 形式
    Liquid
  • 补充说明

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Oncogene PIM 1
    • Oncogene PIM1
    • PIM
    • PIM 1
    • Pim 1 kinase
    • pim 1 kinase 44 kDa isoform
    • Pim 1 oncogene
    • pim 1 oncogene (proviral integration site 1)
    • PIM1
    • pim1 kinase 44 kDa isoform
    • PIM1_HUMAN
    • Pim2
    • PIM3
    • Proto oncogene serine/threonine protein kinase Pim 1
    • Proto-oncogene serine/threonine-protein kinase Pim-1
    • Proviral integration site 1
    • Proviral integration site 2
    see all
  • 功能
    May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • 组织特异性
    Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated on both serine/threonine and tyrosine residues.
  • 细胞定位
    Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt

图片

  • Sample Kinase Activity Plot.
  • ab60835 on SDS-PAGE, MW ~62kDa.

文献

ab60835 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. Here are my answers to your questions. Is there a loading limit (cell number) for sample buffer as the lysis buffer? We recommend 1 ml of lysis buffer per 10 million cells. For sample buffer, if you mean gel loading b...

Read More

Thank you for your call yestserday and for your patience while I have looked into your enquiry. This protein has not been pre-boiled, so to run a standard reduced, denatured Western blot, you can add the PIM1 protein to loading buffer such as Laemmli a...

Read More

The Pim1 antibodies we have in our catalog are : ab54457 : the epitope recognized by this antibody maps to a region between aa 250-313 and is specific to Pim-1. No cross-reactivity with Pim-2 or -3 is expected. ab74047 and ab74118 can't react with ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册