Recombinant人PIM1 protein (ab60835)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

相关产品

技术指标

Our Abpromise guarantee covers the use of ab60835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • 形式Liquid
  • 补充说明

    ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Oncogene PIM 1
    • Oncogene PIM1
    • PIM
    • PIM 1
    • Pim 1 kinase
    • pim 1 kinase 44 kDa isoform
    • Pim 1 oncogene
    • pim 1 oncogene (proviral integration site 1)
    • PIM1
    • pim1 kinase 44 kDa isoform
    • PIM1_HUMAN
    • Pim2
    • PIM3
    • Proto oncogene serine/threonine protein kinase Pim 1
    • Proto-oncogene serine/threonine-protein kinase Pim-1
    • Proviral integration site 1
    • Proviral integration site 2
    see all
  • 功能May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • 组织特异性Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on both serine/threonine and tyrosine residues.
  • 细胞定位Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt

Recombinant human PIM1 protein 图像

  • Sample Kinase Activity Plot.
  • ab60835 on SDS-PAGE, MW ~62kDa.

Recombinant human PIM1 protein (ab60835)参考文献

ab60835 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Here are my answers to your questions. Is there a loading limit (cell number) for sample buffer as the lysis buffer? We recommend 1 ml of lysis buffer per 10 million cells. For sample buffer, if you mean gel loading b...

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Thank you for your call yestserday and for your patience while I have looked into your enquiry. This protein has not been pre-boiled, so to run a standard reduced, denatured Western blot, you can add the PIM1 protein to loading buffer such as Laemmli a...

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The Pim1 antibodies we have in our catalog are : ab54457 : the epitope recognized by this antibody maps to a region between aa 250-313 and is specific to Pim-1. No cross-reactivity with Pim-2 or -3 is expected. ab74047 and ab74118 can't react with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"