Recombinant人Phospholipase A2 X protein (His tag) (ab63847)

概述

  • 产品名称
    Recombinant人Phospholipase A2 X protein (His tag)
  • 蛋白长度
    Full length protein

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MRGSHHHHHHGMASHMGILELAGTVGCVGPRTPIAYMKYGCFCGLGGHGQ PRDAIDWCCHGHDCCYTRAEEAGCSPKTERYSWQCVNQSVLCGPAENKCQ ELLCKCDQEIANCLAQTEYNLKYLFYPQFLCEPDSPKCD
    • 分子量
      16 kDa including tags
    • 氨基酸
      43 to 165
    • 标签
      His tag N-Terminus
    • 额外的序列信息
      Mature chain without signal peptide, without propeptide.

技术指标

Our Abpromise guarantee covers the use of ab63847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明
    Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.01M Tris buffer, pH 7.2

  • 复溶
    Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.

常规信息

  • 别名
    • Group 10 secretory phospholipase A2
    • Group X secretory phospholipase A2
    • GX sPLA2
    • GXPLA2
    • GXSPLA2
    • PA2GX_HUMAN
    • Phosphatidylcholine 2 acylhydrolase GX
    • Phosphatidylcholine 2-acylhydrolase 10
    • Phospholipase A2 group X
    • PLA2G10
    • SPLA2
    • sPLA2-X
    • sPLA2X
    see all
  • 功能
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
  • 组织特异性
    Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
  • 序列相似性
    Belongs to the phospholipase A2 family.
  • 细胞定位
    Secreted.
  • Information by UniProt

Recombinant Human Phospholipase A2 X protein (His tag) 图像

  • 12% SDS-PAGE separation of ab63847. Molecular Weight: ~15.5 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

Recombinant Human Phospholipase A2 X protein (His tag) (ab63847)参考文献

ab63847 has not yet been referenced specifically in any publications.

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