Recombinant人PGAM1 protein (ab100885)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP18669
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
    • 分子量31 kDa including tags
    • 氨基酸1 to 254
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab100885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab100885 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0% None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • BPG dependent PGAM 1
    • BPG dependent PGAM1
    • BPG-dependent PGAM 1
    • OTTHUMP00000059414
    • PGAM 1
    • PGAM A
    • PGAM B
    • PGAM-B
    • PGAM1
    • PGAM1_HUMAN
    • PGAMA
    • PGAMB
    • Phosphoglycerate mutase 1
    • Phosphoglycerate mutase 1 brain
    • Phosphoglycerate mutase A
    • Phosphoglycerate mutase A nonmuscle form
    • Phosphoglycerate mutase isozyme B
    see all
  • 功能Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 组织特异性In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 序列相似性Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt

Recombinant Human PGAM1 protein 图像

  • ab100885 (3 µg) on an SDS-PAGE gel (15%).
  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
    Lane 2 : Recombinant Human PGAM1 protein (ab100885) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Recombinant Human PGAM1 protein (ab100885)参考文献

ab100885 has not yet been referenced specifically in any publications.

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