The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: approximately 82-83 pmole/min/µg.
Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute
% SDS-PAGE. ab85559 was purified by using conventional chromatography techniques
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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.