Recombinant人Peroxiredoxin 2 protein (ab79947)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab79947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 149 pmol/min/µg. 3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Epididymis secretory sperm binding protein Li 2a
    • HEL S 2a
    • MGC4104
    • Natural killer cell enhancing factor B
    • Natural killer cell-enhancing factor B
    • Natural Killer Enhancing Factor B
    • NKEF B
    • NKEF-B
    • NKEFB
    • Peroxiredoxin-2
    • PRDX 2
    • PRDX2
    • PRDX2_HUMAN
    • PrP
    • PRX2
    • PRXII
    • PTX1
    • TDPX1
    • Thiol Specific Antioxidant 1
    • Thiol specific antioxidant protein
    • Thiol-specific antioxidant protein
    • Thioredoxin Dependent Peroxide Reductase 1
    • Thioredoxin peroxidase 1
    • Thioredoxin-dependent peroxide reductase 1
    • Torin
    • TPX1
    • TSA
    see all
  • 功能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human Peroxiredoxin 2 protein 图像

  • Lane 1: ab79947 on 14% SDS-PAGE, Coomassie staining, 10µg.
    Lane 2: Protein marker.
  • Anti-Peroxiredoxin 2 antibody (ab86295) at 1 µg/ml + Recombinant human Peroxiredoxin 2 protein (ab79947) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

Recombinant human Peroxiredoxin 2 protein (ab79947)参考文献

ab79947 has not yet been referenced specifically in any publications.

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