Recombinant人Peroxiredoxin 1 protein (ab79945)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab79945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Heme binding 23 kDa protein
    • MSP23
    • Natural killer cell-enhancing factor A
    • NKEF A
    • NKEF-A
    • NKEFA
    • OSF3
    • Osteoblast specific factor 3
    • PAG
    • Paga
    • PAGB
    • Peroxiredoxin-1
    • PRDX1
    • PRDX1_HUMAN
    • Proliferation associated gene A
    • Proliferation-associated gene protein
    • PRX1
    • PrxI
    • TDPX2
    • Thioredoxin peroxidase 2
    • Thioredoxin-dependent peroxide reductase 2
    see all
  • 功能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • 序列相似性Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 翻译后修饰Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant human Peroxiredoxin 1 protein 图像

  • Lane 1: ab79945 on 14% SDS-PAGE, Coomassie staining, 20µg.
    Lane 2: Protein marker.

Recombinant human Peroxiredoxin 1 protein (ab79945)参考文献

ab79945 has not yet been referenced specifically in any publications.

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