重组人Peroxiredoxin 1/PAG蛋白(ab74172)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Peroxiredoxin 1/PAG蛋白
参阅全部 Peroxiredoxin 1/PAG 蛋白酶 -
生物活性
Specific activity: approximately 600-670 pmole/min/µg. Enzymatic activity is defined as the amount of hydroperoxide that 1µg of enzyme can reduce at 25C for 1 minute. Conditions - Reaction buffer : 1mM DTT, 0.03X PBS, 0.5% glycerol. - Initial amount of substrate : 0.85µg - Total reaction volume: 100 µl. - Reaction temperature: Room temperature Specific activity: approximately 600-670 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX1 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute.
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纯度
> 90 % SDS-PAGE.
ab74172 is purified by conventional chromatography techniques. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab74172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.242% Tris, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Heme binding 23 kDa protein
- MSP23
- Natural killer cell-enhancing factor A
see all -
功能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻译后修饰
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab74172 被引用在 2 文献中.
- Li P et al. LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy. EMBO Mol Med 14:e14511 (2022). PubMed: 34779136
- Chung MC et al. S-nitrosylation of peroxiredoxin 1 contributes to viability of lung epithelial cells during Bacillus anthracis infection. Biochim Biophys Acta Gen Subj 1861:3019-3029 (2017). PubMed: 27612662